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Theme - Multifunctional exosome-mimetics for targeted anti-glioblastoma therapy by manipulating protein corona.
To study the formation of the protein corona and its effect on the cellular uptake of biomimetic nanoparticles modified with chimeric proteins, BBB penetration, targeting ability for precise drug delivery to the brain
Research and development of delivery systems to improve the therapeutic index while reducing the side effects of drugs from brain tumors.
Multifunctional exosomes-mimetics are developed and decorated with angiopep-2.
It has been reported that exosomes, endogenous nano-sized vesicles, released by almost all living cells, may have abilities to cross biological barriers such as BBB, offering efficient brain drug delivery
Owing to the nanosize and lipid bilayer membranes of exosomes, liposomes hold great potentials for mimicking exosomal structure.
U87-MG cells-derived exosomes were prepared from supernatant of cells culture.
Liposomes (Lipo) were synthesized by the thin layer evaporation followed by the extrusion method.
To form protein corona in vitro, nanoparticles were incubated directly with fetal bovine serum at 37℃ for 1h.
Biodistribution and antitumor studies were performed in vivo in mice with GBM.
Results demonstrated that EM could mimic exosomes closely in regards to size and protein markers.
Collectively, these results demonstrated that EM absorbed fewer proteins than Lipo and Ang modification has little influence in the protein corona formation.
Free DTX exhibited limited suppression of tumor growth in GBM-bearing mice, while DTX@Ang-EM showed the most significant suppression of GBM growth
In this study, they developed an EM by integrating cell membrane proteins into liposomes as a biomimetic drug delivery system for brain tumor drug delivery.
Ang-EM provided a promising delivery platform for targeted brain drug delivery and GBM therapy.